Journal: Clinical Epigenetics

Article Title: Exploration of hydroxymethylation in Kagami-Ogata syndrome caused by hypermethylation of imprinting control regions

doi: 10.1186/s13148-015-0124-y

Figure Lengend Snippet: Methylation/hydroxymethylation analysis by BS and oxBS treatment at the IG-DMR. a Methylation values (%methylation) determined by pyrosequencing with BS or oxBS conversion for three KOS14 blood samples (Pt1–Pt3), one control pooled blood sample, and one control adult brain sample. Solid lines denote values with BS, whereas dotted lines denote those with oxBS. b Methylation profiles determined by cloning-based sequencing with BS or oxBS conversion for one KOS14 blood sample (Pt1), one control blood, and one control adult brain sample. For BS, filled and open circles indicate (methylated + hydroxymethylated) and unmethylated cytosines at the CpG sites, respectively, while for oxBS, filled and open circles indicate methylated and (hydroxymethylated + unmethylated) cytosines at the CpG sites, respectively. The typing data of A/G SNP (rs10133627) are also denoted. Note this control blood sample was derived from a single person, rather than a pooled blood sample

Article Snippet: In this study, we analyzed the 5hmC level and distribution pattern using the newly developed oxBS treatment combined with pyrosequencing, cloning-based sequencing, and the 450K methylation array in peripheral blood taken from three patients with KOS14 and normal controls, and in the brain sample from the normal control.

Techniques: Methylation, Control, Cloning, Sequencing, Derivative Assay

Journal: Clinical Epigenetics

Article Title: Exploration of hydroxymethylation in Kagami-Ogata syndrome caused by hypermethylation of imprinting control regions

doi: 10.1186/s13148-015-0124-y

Figure Lengend Snippet: Representative data of BS/oxBS-array. a Density plots of normalized beta values with BS and oxBS conversion for three KOS14 blood samples (Pt1–Pt3), one control pooled blood sample, and one control adult brain sample. Beta values show a bimodal distribution for both BS and oxBS conversion samples. Of note, a left-skewed methylated peak is observed in the oxBS brain sample. b Box and whisker plots of Δ β score (corresponding to 5hmC value) for each sample. c Distribution of probes with Δ β ≥ 0.1 according to genomic features for each sample

Article Snippet: In this study, we analyzed the 5hmC level and distribution pattern using the newly developed oxBS treatment combined with pyrosequencing, cloning-based sequencing, and the 450K methylation array in peripheral blood taken from three patients with KOS14 and normal controls, and in the brain sample from the normal control.

Techniques: Control, Methylation, Whisker Assay